Soil and root sampling method for Phytophthora agathidicida detection

Standard operating procedure for sampling roots and soil in the field for the purpose of Phytophthora agathidicida (PA) detection.

Purpose

This document describes the standardised and approved operating procedure for sampling roots and soil in the field for the purpose of Phytophthora agathidicida (PA) detection.

Soil and roots obtained using this method may be used to amplify PA using the standard soil baiting method, followed by downstream analysis using the loop-mediated isothermal amplification (LAMP) or morphological assessment.